Helicobacter pylori is a bacteria infection and one of the major causes of gastritis and peptic ulcer. Pertaining to early diagnosis of the infection, no single test alone can be used as the best defection method for Helicobacter pylori. However, the best detecting methods could be gram reaction, serology and PCR which detected H. pylori in 133 of the 532 patients who underwent microscopy in three hospitals in western Nigeria in the course of investigations for pyspesia between 1999 and 202. Culture alone, detecting only 5, stands out since it involves isolation of the organism specifically. Isolates subjected to in vitro antibiotic susceptibility in a test showed resistance to amoxicillin, cytomycin, metronidazole, tetracycline, and piperacilin, but were sensistive to morfloxacin, cirofloxacine, ofloxacin and clarithromycin.